Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher
Date created: 2016-10-13 11:00:00
Date modified: 2016-12-02 08:37:33
AzF-Synthetase for use in E.coli
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2020043_sequence (Version 1) |
Description
This is the 4-Azido-L-phenylalanine-synthetase to be used as a orthogonal synthetase in E.coli. This part can be used together with the [[Part:BBa_K2020042|cognate tRNA BBa_K2020042]] to incorporate AzF in response to an amber stop codon.Notes
Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs.Team Aachen 2016 used similar synthetases in pACYC with p15A-ORI and Gent-Resistance. Together with a tRNA (BBa_K2020042) with own Ipp-Promoter and Terminator.