Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_K2020052_sequence (Version 1)
|
Description
This is a DMNBS-synthetase to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate DMNBS in response to an amber stop codon.
Notes
The synthetase was created via mutation library following computational design from Methanococcus janaschii wild type tyrosyl synthetase.
Source
Basis for mutation was Methanococcus janaschii wild type tyrosyl synthetase.