BBa_K2020054

BBa_K2020054 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2020054

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher

Date created: 2016-10-14 11:00:00

Date modified: 2016-12-02 08:53:18

DMNBS-Synthetase for use in E.coli

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2020054_sequence (Version 1)

Description

This is a DMNBS-synthetase to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate DMNBS in response to an amber stop codon.

Notes

The synthetase was created via mutation library following computational design from Methanococcus janaschii wild type tyrosyl synthetase.

Source

Basis for mutation was Methanococcus janaschii wild type tyrosyl synthetase.

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2020054/1

Member of these Collections

• iGEM Parts Registry