BBa_K2027054

BBa_K2027054 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2027054

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Gordon Sun

Date created: 2016-10-23 11:00:00

Date modified: 2016-10-24 02:36:40

H. Brasiliensis HRT1 cDNA (codon Optimized)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2027054_sequence (Version 1)

Description

This sequence contains the Hevea brasiliensis??HRT1??mRNA for cis-prenyltransferase, complete cds. The sequence has been codon optimized for expression in E. coli. HRT1 is a cis-prenyltransferase and is responsible for initialization and elongation of polyisoprene polymers. It takes in IPP or DMAPP and polymerizes it to a polyisoprene chain.

Notes

This sequence has been codon optimized for expression in E. coli.

No other modifications have been made to the sequence.

Source

Sourced from Asawatreratanakul K, Zhang YW, Wititsuwannakul D, et al. Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis. Eur J Biochem. 2003;270(23):4671-80. (http://www.ncbi.nlm.nih.gov/nuccore/AB061234?report=GenBank)

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2027054/1

Member of these Collections

• iGEM Parts Registry