BBa_K2036018

BBa_K2036018 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2036018
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhangyu Cheng
Date created: 2016-09-19 11:00:00
Date modified: 2016-10-10 01:53:40

pRE-RBS-Cro-RBS-CII-TT-ptrp2



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K2036018_sequence (Version 1)

Description

pRE-RBS-Cro-RBS-CII-TT

Notes

This is Cro and CII tandom expression. CII expressed at the downtream of pRE can form a positive feedback to enhance Cro's expression to reach a ideal exppression level.

Source

pRE, Cro and CII are all from bacteriaphage lambda.

Sequence Annotation Location Component / Role(s)
pRE
RBS core
Cro
RBS core
CII
terminator
scar
ptrp2
1,52
53,64
75,274
275,286
293,586
587,715
716,721
722,806
promoter feature/promoter
feature/rbs ribosome_entry_site
feature/cds CDS
ribosome_entry_site feature/rbs
feature/cds CDS
stem_loop feature/stem_loop
feature/scar
feature/promoter promoter
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2036018/1