BBa_K2036019

BBa_K2036019 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2036019

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zhangyu Cheng

Date created: 2016-09-19 11:00:00

Date modified: 2016-09-24 05:08:21

RFP-LAAtag-TT-pRM-RBS-GFP-LVAssrAtag

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K2036019_sequence (Version 1)

Description

ptrp-RBS-CI-TT-pR-RBS-CIII

Notes

With supplimentory Trp in the media, Ptrp can be silenced and when we add IAA to induce it, the downstream gene CI will be produced to block pR thus there will be no CIII produced.

Source

ptrp are mutant stype from HUST-China previous project. CI, CIII and pR are all derived from bacteriaphage lambda.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS core	1,12	ribosome_entry_site, feature/rbs
BBa_K1399002	19,718	sequence_feature, feature/misc
pRM	903,939	promoter, feature/promoter
RBS core	940,951	feature/rbs, ribosome_entry_site
GFP	958,1671	feature/cds, CDS
LVAssrAtag	1672,1713	tag, feature/tag

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2036019/1

Member of these Collections

• iGEM Parts Registry