BBa_K2036021

BBa_K2036021 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2036021
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhangyu Cheng
Date created: 2016-09-19 11:00:00
Date modified: 2016-09-24 01:11:15

placm-pRE-RBS-Cro-RBS-CII-TT



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K2036021_sequence (Version 1)

Description

pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAtag-TT-pRM-RBS-GFP-LVAtag

Notes

It is a tri-stable system with a certain threshold and it functions as a basic gene expression toolkit for two input and three related output.

Source

The circuit are redesign of bacteriaphage lambda operon.

Sequence Annotation Location Component / Role(s)
BBa_M36138
Interval sequence
pRE
RBS core
Cro
RBS core
Interval sequence
CII
TT
1,245
246,251
252,303
304,318
319,525
526,537
538,543
544,837
838,966
feature/BioBrick engineered_region
feature/misc sequence_feature
promoter feature/promoter
ribosome_entry_site feature/rbs
CDS feature/cds
ribosome_entry_site feature/rbs
sequence_feature feature/misc
CDS feature/cds
feature/stem_loop stem_loop
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2036021/1