BBa_K2036022

BBa_K2036022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2036022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhangyu Cheng
Date created: 2016-09-19 11:00:00
Date modified: 2016-09-24 01:57:00

patp2-RBS-CI-TT-pR-RBS-CIII



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K2036022_sequence (Version 1)

    Description

    placm-pRE-RBS-Cro-RBS-CII-TT

    Notes

    With this two pormoter design, lactose an induce downstream gene's expression,and CII protein can form a positive feedback by activate pRE so, the circuit can fastly reach a stable state for Cro expression.

    Source

    placm is a mutant lac promoter which has been deleted a illegal restriction site. pRE, Cro, CII are from bacteriaphage lambda.

    Sequence Annotation Location Component / Role(s)
    patp2(RBS included)
    CI
    Terminator
    pR
    RBS
    CIII
    		1,373
    374,1087
    1088,1216
    1217,1310
    1311,1328
    1329,1493
    		feature/promoter promoter
    feature/cds CDS
    stem_loop feature/stem_loop
    feature/promoter promoter
    ribosome_entry_site feature/rbs
    feature/cds CDS

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2036022/1
     

    Attachments

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