BBa_K2036025

BBa_K2036025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2036025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zhangyu Cheng

Date created: 2016-09-19 11:00:00

Date modified: 2016-09-24 03:13:03

Kozak-Snrk2.2-TTADH1-pCyc

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K2036025_sequence (Version 1)

Description

placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase

Notes

It is a lactic acid balance circuit in E.coli with lactose signal to initiate. And when the pH environment are within 7 to 9,the host strain will prepare beta-galactosidase for next lactose input.

Source

derived from bacteriaphage lambda operon. And placm, iLDH, patp2 are from the Registry. Beta-galactosidase coding sequence are reffered to pUC119 commerial vector

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Kozak	1,12	ribosome_entry_site, feature/rbs
SnRK2.2	13,1101	feature/cds, CDS
BBa_K801012	1102,1450	engineered_region, feature/BioBrick
BBa_I766555	1451,1694	feature/BioBrick, engineered_region

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2036025/1

Member of these Collections

• iGEM Parts Registry