Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maxence Pfeiffer
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 08:39:06
Coding sequences of proteins FRB* and GFP11 (subunit of tripartite GFP)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K2039004_sequence (Version 1) |
Description
This part is the complementary part of Bba_K2039003.The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the medium. It is particularly useful for protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria.
FKBP protein is linked to GFP 10.
The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).