Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Thiban Navaratnarajah
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 12:06:55
MelA P334S-Mutant tyrosinase gene heightened expression
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2045004_sequence (Version 1) |
Description
iGEM Concordia has showcased the synthesis of nanoparticles through many chemical and plant based methods. iGEM Concordia wanted to find an innovative application where we will be able to develop a system where we can synthesize and attach nanoparticles through a single biological cell system.MelA is a gene that produces Eumelanin, which is a dark pigment. Eumelanin has carboxyl and hydroxyl quinone groups, whose charges help reduce gold ions, present in the cytoplasm, into gold nanoparticles. In order to accomplish this, we used iGEM Cambridge???s part, BBa_K274001, for nanoparticle production.
In order to make more gold nanoparticles within the E.coli cell, iGEM Concordia IMPROVED the part in order to have higher expression of MelA for higher gold nanoparticle synthesis. This improvement was done by causing a point mutation.
Notes
A point mutation was needed to be made through PCR in order to ensure an increase in expression.Source
iGEM Cambridge???s part, BBa_K274001Rhizobium etli CFN42