BBa_K2047001

BBa_K2047001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2047001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yu Jiang

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-21 12:37:05

Fluorescent reporter system with stem-loop, GFP_Stem-loop with free energy of -25.6 kcal/mol

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K2047001_sequence (Version 1)

Description

This sequence encodes a stem loop that can regulate multiple gene expression when inserted in intergenic region followed with a RNaseE site in E.coli. Different stem loops have different regulation effects and we quantitatively weighed this difference by their free energy evaluated by a software---Mfold .Of which the free energy of the most stable secondary structure of this sequence is -25.6 kcal/mol.

Notes

none

Source

no

Components

Access	Instance	Definition
public	BBa_E0040	GFP
public	BBa_K2047009	BBa_K2047009

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_E0040	1,720	GFP
BBa_K2047009	721,801	BBa_K2047009

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2047001/1

Member of these Collections

• iGEM Parts Registry