BBa_K2047003

BBa_K2047003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2047003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yu Jiang

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-21 12:39:24

Fluorescent reporter system with stem-loop of -38.7 kcal/mol, GFP_stem-loop

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K2047003_sequence (Version 1)

Description

This sequence encodes a stem loop that can regulate multiple gene expression when inserted in intergenic region followed with a RNaseE site in E.coli. Different stem loops have different regulation effects and we quantitatively weighed this difference by their free energy evaluated by a software---Mfold .Of which the free energy of the most stable secondary structure of this sequence is -20 kcal/mol.

Notes

none

Source

no

Components

Access	Instance	Definition
public	BBa_E0040	GFP
public	BBa_K2047004	BBa_K2047004

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_E0040	1,720	GFP
BBa_K2047004	721,809	BBa_K2047004

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2047003/1

Member of these Collections

• iGEM Parts Registry