GroEL

BBa_K205000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K205000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michael James George Milevskiy

Date created: 2009-09-07 11:00:00

Date modified: 2015-05-08 01:11:23

GroEL is a chaperone protein that prevents polypeptide misfolding.

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K205000_sequence (Version 1)

Description

GroEL is a chaperone protein that is responsible for the prevention of polypeptide mis-folding. In conjunction with GroES, these two subunits form an assembly dimer that are promote efficient protein folding, especially under stressful conditions such as heat shock. This chaperone protein belongs to the eukaryotic HSP60 (heat shock protein) family.

http://www.uniprot.org/uniprot/P0A6F5

Notes

Gene was provided by Christos Kyratsous, we used mutagenesis to remove the internal restriction site, EcoR1, BamH1

Source

Christos Kyratsous
Columbia Univ, Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA

Ref: Kyratsous, CA, Silverstein, SJ, DeLong, CR, Panagiotidis, CA. (2009) "Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli" GENE VOL: 440 Issue: 1-2 Pages: 9-15 Date: Jul 1 2009

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start of GroEL	1,3	feature/start, start_codon
End of GroEL	1597,1599	stop_codon, feature/stop
Mutation from t to c to remove BamHI restriction site	1485,1485	point_mutation, feature/s_mutation

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K205000/1

Member of these Collections

• iGEM Parts Registry