DnaJ/GrpE

BBa_K205003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K205003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Richard Schlegel

Date created: 2009-09-27 11:00:00

Date modified: 2015-05-08 01:11:23

DnaJ and GrpE are chaperone proteins that assists with polypeptide folding

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K205003_sequence (Version 1)

Description

In conjuction with DnaK and GrpE, DnaJ assists in correct polypeptide folding and is the Prokaryotic homologue of the Eukaryotic chaperone system 'Hsp70'. This gene, along with DnaK, GrpE, GroEL and GroES forms the heat shock system in bacteria.

Note that GrpE is used to catalyse the dephosphorylation of ATP to ADP, and changes the protein conformation of the DnaK/DnaJ complex to faciliate efficient polypeptide folding.

Notes

There were no other considerations that had to be taken into account when designing this part.

Source

Christos Kyratsous Columbia Univ, Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA

Ref: Kyratsous, CA, Silverstein, SJ, DeLong, CR, Panagiotidis, CA. (2009) "Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli" GENE VOL: 440 Issue: 1-2 Pages: 9-15 Date: Jul 1 2009

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start of GrpE	1,3	start_codon, feature/start
Start of DnaJ	798,800	feature/start, start_codon
End of GrpE	592,594	stop_codon, feature/stop
End of DnaJ	1839,1841	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K205003/1

Member of these Collections

• iGEM Parts Registry