BBa_K2050422

BBa_K2050422 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2050422
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Muhammad Farhan Maruli
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 10:42:32

Mouse H1 promoter (RNA Polymerase III promoter)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K2050422_sequence (Version 1)

Description

RNA polymerase III is responsible for transcribing small RNA molecules including the 5S rRNA and tRNAs. RNA polymerase III promoters, including the H1 promoter, is useful in creating synthetic functional RNA-encoding genes. RNA polymerase III promoters are considerably smaller than RNA polymerase II promoters and initiates expression at ten times the rate RNA polymerase II promoters do. Examples on using functional RNA genes include miRNA expression, guide RNA expression for CRISPR technology, and immunostimulatory RNA (isRNA) expression, the latter used in many projects (including ours) on vaccination.

The use of RNA molecules as a vaccine adjuvant hinges on the interaction of those RNA molecules with the retinoic acid-inducible gene 1 (RIG-1) receptor, which is a pathogen-associated molecular pattern recognition receptor specialized in detecting non-self RNA molecules and triggering the innate immunity upon recognizing such molecules. The presence of 5'-PPP on these molecules distinguish them from self RNA molecules, in which the 5'-PPP is usually moved or masked. Because RNA polymerase III transcription does not initiate 5-capping like RNA polymerase II transcription does, the use of RNA polymerase III promoters have this as an additional advantage for isRNA expression. The use of a mouse H1 promoter instead of that of human is preferable for human applications as it limits the probability for homologous recombination with the human genome.

Notes

None

Source

The sequence was obtained from Myslinski et al. (doi:10.1093/nar/29.12.2502).

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2050422/1