Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Carmen Padilla, Melissa Gonzalez & Melissa Rios
Date created: 2016-09-27 11:00:00
Date modified: 2016-09-28 06:11:09
LacI for Acidithiobacillus
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2055007_sequence (Version 1) |
Description
Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron. LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans.Notes
It does not have a promoter.Source
The RBS used is the BBa_B0034, the terminator is the BBA_B0012, the LacI reculated Promoter is the BBa_R0011.The sequence for the FUR Box was obtained from the results of the paper of Quatrini et al. (Citation in our wiki), choosing the Fur box with the best score.
The sequence for the FUR protein was obtained from the Acidithiobacillus ferrooxidans genome.