BBa_K2057006

BBa_K2057006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2057006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jos?? Eleazar Barboza Corona

Date created: 2016-10-16 11:00:00

Date modified: 2016-10-17 02:07:21

Expression of gfp under the regulation of promoter BtI-BtII

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K2057006_sequence (Version 1)

Description

We made this composite part to check the functionality of the promoter BtI-BtII in Escherichia coli. Biobrick BBa_K2057001 (promoter BtI-BtII) includes the promoter sequence and the ribosome binding sequence.

Notes

As promoter BtI-BtII is not available in the biobrick we amplify from the cry1Ac gene

Source

The promoter BtI-BtII is located on a megaplasmid of Bacillus thuringiensis and control the expression of cry1Ac. gfp is obtained from the biobrick BBa_E0040

Components

Access	Instance	Definition
public	BBa_K2057001	BBa_K2057001
public	BBa_E0040	GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2057001	1,387	BBa_K2057001
BBa_E0040	394,1113	GFP

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2057006/1

Member of these Collections

• //chassis/eukaryote
• iGEM Parts Registry