BBa_K2060002

BBa_K2060002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2060002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cardiff_Wales Team, Geraint Parry

Date created: 2016-10-05 11:00:00

Date modified: 2016-10-06 06:48:17

LUXoperon with mKeima

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K2060002_sequence (Version 1)

Description

The pBAD::LUXoperon (BBa_K325909) has been well characterised and the bacteria containing this biobrick is able to produce blue light in vivo after induction by Arabinose.

We have generated a part that has RBS-mKeima at the 3' end of the LUXoperon in order to attempt to bring about a stroke shift in light output from blue>red.

Notes

We used a longer RBS sequence upstream of mKeima to enable co-translation of mKeima along with the components of the LUXoperon

Source

rbs-mKeima sequence was synthesised and added to BBa_K325909 by Gibson assembly.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LUXoperon BBa_K325909	1,7639	engineered_region, feature/BioBrick
RBS	7646,7658	feature/rbs, ribosome_entry_site
mKeima	7665,8333	CDS, feature/protein

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2060002/1

Member of these Collections

• iGEM Parts Registry