Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-13 11:48:44
Constitutive Lac Repressor on UNS Standard
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2066016_sequence (Version 1) |
Description
to be replacedNotes
UNS standard is used to allow for easy PCR Amplification using standard primers.Source
This parts was prepared using DNA synthesis by IDT. The UNS2 and UNS3 sequences are taken from Torella et al. 2013 and are ideal for PCR based cloning.Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
| Access | Instance | Definition |
| public public public public public public | BBa_K2066018 BBa_J23101 BBa_B0034 BBa_K2066524 BBa_B0015 BBa_K2066019 | BBa_K2066018 BBa_J23101 BBa_B0034 BBa_K2066524 BBa_B0015 BBa_K2066019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2066018 BBa_J23101 BBa_B0034 BBa_K2066524 BBa_B0015 BBa_K2066019 | 1,40 41,75 76,87 88,1179 1180,1308 1309,1348 | BBa_K2066018 BBa_J23101 BBa_B0034 BBa_K2066524 BBa_B0015 BBa_K2066019 |