Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-28 08:42:17
pACT-Tet on UNS Standard
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_K2066037_sequence (Version 1) |
Description
This part is part of William and Mary iGEM 2016's library of IPTG-inducible RBS characterization parts. It contains the RBS BBa_B0031.The part codes for the expression of a superfolder GFP and is regulated by a lacI-repressible plLacO-1 Promoter. By adding IPTG one should be able to induce the expression of sfGFP and compare the induction curve to other IPTG-inducible RBS characterization parts from our library to determine the relative strengths of different RBS sequences across an induction curve.
We have included the self-cleaving ribozyme RiboJ immediately upstream of the RBS sequence in order to buffer against translational influence from the 5' untranslated region conferred to the transcript by the promoter sequence.
Notes
See Design Details for individual parts plLacO-1 (K2066508) and sfGFP (K2066509).Source
This part was assembled from existing BioBrick parts.| Access | Instance | Definition |
| public public public | BBa_K2066018 BBa_K2066533 BBa_K2066019 | BBa_K2066018 BBa_K2066533 BBa_K2066019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2066018 BBa_K2066533 BBa_K2066019 | 1,40 41,2994 2995,3034 | BBa_K2066018 BBa_K2066533 BBa_K2066019 |