BBa_K2066500

BBa_K2066500 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K2066500

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss

Date created: 2016-07-11 11:00:00

Date modified: 2016-07-12 10:18:00

UNS 2 Sequence, from Torella et al., 2013

Details

• Types: DnaRegion
• Roles: Primer; primer
• Sequences: BBa_K2066500_sequence (Version 1)

Description

This is Unique Nucleotide Sequence 2, (UNS 2), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki.

Notes

UNS 2 was chosen because it works well with UNS 3 and it is in accordance with the BioBrick standard.

Source

Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2066500/1

Member of these Collections

• iGEM Parts Registry