BBa_K2066506

BBa_K2066506 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2066506
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Likhitha Kolla
Date created: 2016-08-30 11:00:00
Date modified: 2016-10-12 12:27:33

RiboJ (Ribozyme Insulator) Lou et. al. 2012



Types
DnaRegion

Roles
sequence_feature

Other

Sequences BBa_K2066506_sequence (Version 1)

Description

RiboJ is the sequence for a ribozyme studied in Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context"). WM iGEM 2016 used this sequence between the promoter and ribosome sequence. One of our goals for using this part is moving it onto a Biobrick backbone. Furthermore, In Lou et. al, this ribozyme sequence was said to act as an insulator which generalizes protein expression levels for a given promoter. We used RiboJ to collect data for our Ribozyme characterization project as well as our ribosome and promoter characterization projects.

Notes

We designed this part to use as an insulator and also move this riboJ sequence onto a Biobrick backbone.

Source

Part sequence is from Lou et al. 2012, Supplemental Section V (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???).

igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K2066506/1