BBa_K2066508

BBa_K2066508 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2066508
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Likhitha Kolla
Date created: 2016-08-30 11:00:00
Date modified: 2016-08-31 09:01:04

Modified pLacO-1 Promoter (Lou et. al 2012)



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K2066508_sequence (Version 1)

Description

This promoter sequence is modified from section V of Supplementary Material of Lou et al. The Supplementary sequence contains 98bp of the end of BioBrick backbone pSB1C3, followed by an EcoRI site and an XbaI site, then 20bp of the beginning of pTac (as described in fig. S1), before beginning the sequence of plLacO-1 (as described in fig. S1). Here we use only the 20bp of pTac followed by the plLacO-1 sequence as described in fig. S1. WM iGEM 2016 used this part for our Ribozyme Characterization project.

Notes

Design inspired by Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???) so that constitutive LacI repressor can bind to it.

Source

Part sequence inspired by Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???)

igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K2066508/1