Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pavle Jeremic
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 03:22:48
A non-specific phosphotransferase, codon optimized for B.subtilis.
| Types | DnaRegion |
| Roles | Coding CDS |
Description
This part codes for a phosphotransferase that is able to de-phosphorylate Erythrose-4-Phosphate. It is not a highly specific phosphotransferase and while it operates on both Erythrose-4-Phosphate, its original designation as a beta-phosphoglucomutase indicates it is relatively non-specific. This may pose an issue for cellular growth. It is recommended to grow the bacteria at a lower temperature to determine if non-specific de-phosphorylation is an issue.Notes
We had two major design considerations when working with this sequence. Firstly we had to ensure that the sequence was codon optimized for eventual expression in Bacillus subtilis. We then also had to ensure that no RFC10 restriction sites were in the submitted sequence. We were able to correct both of these issues simultaneously using IDT???s codon optimization software.Source
This part comes from the genome of Thermotoga maritima , and is normally used in T.maritima as a beta-phosphoglucomutase, however later study showed the protein to be highly effective at dephosphorylating Erythrose-4-Phosphate.https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0458-y