Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tong Tong
Date created: 2016-10-16 11:00:00
Date modified: 2016-10-18 02:43:32
Cry4Aa+2A+Cyt1 expressed in C. reinhardtii
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K2074003_sequence (Version 1) |
Description
This devise is consisted of a HSP70A-RBCS2 promoter, Cry4Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.Notes
noneSource
HSP70A-RBCS2 promoter cloned from Chlamydomonas reinhardtiiCry4Aa and Cyt cloned from Bacillus thuringiensis serovar israelensis strain BRC-LLP29, codon optimal for Chlamydomonas reinhardtii
Extended FMDV 2A peptides cloned from FMDV, codon optimal for Chlamydomonas reinhardtii
| Access | Instance | Definition |
| public public public public | BBa_K2074017 BBa_K2074018 BBa_K2074027 BBa_K2074019 | BBa_K2074017 BBa_K2074018 BBa_K2074027 BBa_K2074019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2074017 BBa_K2074018 BBa_K2074027 BBa_K2074019 | 1,496 505,649 656,5104 5113,5346 | BBa_K2074017 BBa_K2074018 BBa_K2074027 BBa_K2074019 |