Intein

BBa_K2075002 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K2075002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Louise Schaar

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-15 02:56:33

Intein

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2075002_sequence (Version 1)

Description

Inteins were integrated into proteins. They useful by the circularization
of a protein by ligating the N-
and C-terminal ends. The team Heidelberg 2014 already used them for thei
r circulation but we
changed the sequence to make the circularization more accurate. They successful showed
that
circularization enhances resistance against proteases and denaturation. We show t
hat circularization
with a TAL-effector makes them more solid and durable for everyday laboratory.
If you want more
Information have a look of our results. The intein sequence have a size of 309
bp.

Notes

No considerations.

Source

tgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaattaa

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Intein	1,309	feature/cds, CDS
Intein Linker	311,353	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2075002/1

Member of these Collections

• iGEM Parts Registry