Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kim L??hmann
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-15 02:17:23
TAL-effector Hax3-2xNN
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2075009_sequence (Version 1) |
Description
This part synthesizes a transcription activator like (TAL) effector protein. They were found in Xanthomonas bacteria. These bacteria are plant pathogenes and infects plants via a secretion typ III system. In the plant cells these proteins binds promoter sites. This leads to a transcriptional activation of the genes behind the promoter.The protein contains tandem repeats up to 34.5. Each repeat binds one nucleotide of the target sequence. The 12th and 13th amino acid of each repeat leads to the specific binding of the DNA sequence.
Notes
We circularised the TAL-effectors with the help of Inteins at the N- and C- terminus of each TAL-effector.The 12th and 13th amino acid of each of the 11.5 repeats from our TAL Hax3 ??? 2xNN are:
NI HD NI HD HD HD NN NN NS HD NI
They bind the DNA sequence: A C A C C C G/A G/A N C A.
Source
They were found in Xanthomonas bacteria. These bacteria are plant pathogenes and infects plants via a secretion type III system. In the plant cells these proteins bind promoter sites. This leads to a transcriptional activation of the genes behind the promoter.| Sequence Annotation | Location | Component / Role(s) |
| SV40 NLS N-Term Hax3 Hax3-2xNN C-Term Hax3 63 | 1,21 22,434 435,1612 1613,1803 | CDS feature/cds feature/cds CDS CDS feature/cds feature/cds CDS |