Intein N-t

BBa_K2075010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2075010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kim Luehmann

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-29 12:33:31

Intein and Intein linker (N-term)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2075010_sequence (Version 1)

Description

Inteins were integrated into proteins. They useful by the circularization of a protein by ligating the N- and C-terminal ends. The team Heidelberg 2014 already used them for their circulation but we changed the linker sequence to make the circularization more accurate. They successful showed that circularization enhances resistance against proteases and denaturation. We show that circularization with a TAL-effector makes them more solid and durable for everyday laboratory. If you want more Information have a look of our results. The intein sequence have a size of 309 bp.

Notes

No considerations

Source

taggcgtatcacgaggcagaatttcagatgatcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttccaactgtttcaatggcggttcgggcggttcgggcggcacaggcggttcgggcggttcgaagactacaagggtggtgcggagtactgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaattaa

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Npu DnaE(C) peptide	1,117	feature/cds, CDS
Linker N-Term	118,162	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2075010/1

Member of these Collections

• iGEM Parts Registry