Intein N-t

BBa_K2075010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2075010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kim Luehmann
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-29 12:33:31

Intein and Intein linker (N-term)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K2075010_sequence (Version 1)

Description

Inteins were integrated into proteins. They useful by the circularization of a protein by ligating the N- and C-terminal ends. The team Heidelberg 2014 already used them for their circulation but we changed the linker sequence to make the circularization more accurate. They successful showed that circularization enhances resistance against proteases and denaturation. We show that circularization with a TAL-effector makes them more solid and durable for everyday laboratory. If you want more Information have a look of our results. The intein sequence have a size of 309 bp.

Notes

No considerations

Source

taggcgtatcacgaggcagaatttcagatgatcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttccaactgtttcaatggcggttcgggcggttcgggcggcacaggcggttcgggcggttcgaagactacaagggtggtgcggagtactgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaattaa

Sequence Annotation Location Component / Role(s)
Npu DnaE(C) peptide
Linker N-Term
1,117
118,162
feature/cds CDS
CDS feature/cds
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Works
 
igem#status
Planning
 
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user/james
 
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user/myers
 
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BBa_K2075010/1