Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haojian Li
Date created: 2016-09-17 11:00:00
Date modified: 2016-09-22 07:47:06
sgRNA generator
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2080000_sequence (Version 1) |
Description
Guide RNA is a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bax, a proapoptosis protein, which can activate the transcription of bax.Notes
We design the sequence of the gRNA that is complementary to the bax promoter region, and try to get closer to the transcription start site to obtain higher activation of bax trascription.Source
BBa_K1179015| Sequence Annotation | Location | Component / Role(s) |
| U6 promoter gRNA scaffold | 1,265 266,365 | promoter feature/promoter sequence_feature feature/misc |