Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haojian Li
Date created: 2016-09-23 11:00:00
Date modified: 2016-09-24 08:41:36
bcl2-sgRNA1
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K2080004_sequence (Version 1) |
Description
This guide RNA is a hundred base-long molecule with a dCas9-KRAB scaffold sequence, which can guide dCas9-KRAB to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bcl-2, a anti-apoptosis protein, which can inhibit the transcription of bcl-2.Notes
We design the sequence of the gRNA that is complementary to the bcl-2 promoter region, and try to get closer to the transcription start site to obtain higher repression of bax trascription.Source
BBa_K2080000| Sequence Annotation | Location | Component / Role(s) |
| U6 promoter gRNA gRNA scaffold | 1,265 266,285 286,367 | promoter feature/promoter sequence_feature feature/misc sequence_feature feature/misc |