BBa_K2080005

BBa_K2080005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2080005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haojian Li
Date created: 2016-09-23 11:00:00
Date modified: 2016-09-24 08:45:04

bcl2-sgRNA2



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K2080005_sequence (Version 1)

Description

This guide RNA is a hundred base-long molecule with a dCas9-KRAB scaffold sequence, which can guide dCas9-KRAB to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bcl-2, a anti-apoptosis protein, which can inhibit the transcription of bcl-2.

Notes

We design the sequence of the gRNA that is complementary to the bcl-2 promoter region, and try to get closer to the transcription start site to obtain higher repression of bax trascription.

Source

BBa_K2080000

Sequence Annotation Location Component / Role(s)
U6 promoter
gRNA
gRNA scaffold
1,265
266,285
286,367
promoter feature/promoter
sequence_feature feature/misc
sequence_feature feature/misc
igem#sampleStatus
Not in stock
igem#status
Planning
 
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2080005/1