BBa_K2080007

BBa_K2080007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2080007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Haojian Li

Date created: 2016-09-23 11:00:00

Date modified: 2016-09-24 09:03:47

bcl2-sgRNA4

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K2080007_sequence (Version 1)

Description

This guide RNA is a hundred base-long molecule with a dCas9-KRAB scaffold sequence, which can guide dCas9-KRAB to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bcl-2, a anti-apoptosis protein, which can inhibit the transcription of bcl-2

Notes

We design the sequence of the gRNA that is complementary to the bcl-2 promoter region, and try to get closer to the transcription start site to obtain higher repression of bax trascription.

Source

BBa_K2080000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
U6 promoter	1,265	feature/promoter, promoter
gRNA	266,285	sequence_feature, feature/misc
gRNA scaffold	286,367	sequence_feature, feature/misc

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2080007/1

Member of these Collections

• iGEM Parts Registry