Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rafael Rojas Obreg??n
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-29 05:58:23
Human thyroxine-binding globulin
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_K2081000_sequence (Version 1) |
Description
There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein, TBG, in serum. It belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined.References:
AUTHORS Ferrara AM, Pappa T, Fu J, Brown CD, Peterson A, Moeller LC, Wyne
K, White KP, Pluzhnikov A, Trubetskoy V, Nobrega M, Weiss RE,
Dumitrescu AM and Refetoff S.
TITLE
JOURNAL J. Clin. Endocrinol. Metab. 100 (1), E173-E181 (2015)
AUTHORS Yan Z, Yan H and Ou H.
TITLE Human thyroxine binding globulin (TBG) promoter directs efficient
and sustaining transgene expression in liver-specific pattern
JOURNAL Gene 506 (2), 289-294 (2012)
Notes
We had to take into account what restriction enzymes could be used in order to further engineer the composed plasmid. We used Xba1, EcoR 1, Spe! and Pst1 because they are compatible with the rest of the plasmids in the registry.Source
TBG complete sequence for plasmid insertion (with Ecor1, Xba1, T4, LacO, RBS: BBA J61110,terminator:BBA_B0015, SPE1, PST)GAATTCTCTAGATAATACGACTCACTATAGGGTGGAATTGTGAGCGGATAACAATTTCTAGAGAAAGAGGCGAATTACTAGATGgagatgcagg tggattcttg ggcatttgct attaacttcc ttccaaaatg tcaccattcc tgtatctggt tctcttggta cttgggcttc atgctacaat ccactgtgca tcacctgaag gcaaagtaac agcctgccat tcatcccaac caaatgccac tctctacaag atgtcatcca ttaatgctga ctttgcattc aatctgtacc ggaggttcac tgtggagacc ccagataaga acatcttctt ttcccctgtg agcatttctg cagctttggt tatgctttcc tttggggcct gctgcagcac ccaaactgag attgtggaga ccttggggtt caacctcaca gacactccaa tggtagagat ccagcatggc ttccagcatc tgatctgttc actgaatttt ccaaagaagg aactggaatt gcagatagga aatgccctct tcattggcaa gcatctgaaa ccactggcaa agttcttgaa tgatgtcaag accctctatg agactgaagt cttttctacc gacttctcca acatttctgc agccaagcag gagattaaca gtcatgtgga gatgcaaacc aaagggaaag ttgtgggtct aattcaagac ctcaagccaa acaccatcat ggtcttagtg aactatattc actttaaagc ccagtgggca aatccttttg atccatccaa gacagaagac agttccagct tcttaataga caagaccacc actgttcaag tgcccatgat gcaccagatg gaacaatact atcacctagt ggatatggaa ttgaactgca cagttctgca aatggactac agcaagaatg ctctggcact ctttgttctt cccaaggagg gacagatgga gtcagtggaa gctgccatgt catctaaaac actgaagaag tggaaccgct tactacagaa gggatgggtt gacttgtttg ttccaaagtt ttccatttct gccacatatg accttggagc cacacttttg aagatgggca ttcagcatgc ctattctgaa aatgctgatt tttctggact cacagaggac aatggtctga aactttccaa tgctgcccat aaggctgtgc tgcacattgg tgaaaaggga actgaagctg cagctgtccc tgaagttgaa ctttcggatc agcctgaaaa cactttccta caccctatta tccaaattga tagatctttc atgttgttga ttttggagag aagcacaagg agtattctct ttctagggaa agttgtgaac ccaacggaag cgtagttggg aaaaaggcca ttggctaatt gcacgtgtgt attgcaatgg gaaataaata aataatatag cctggtgtga ttgatgtgag cttggacttg cattccctta tgatgggatg aagattgaac cctggctgaa ctttgttggc tgtggaagag gccaatccta tggcagagca ttcagaatgt caatgagtaa ttcattatta tccaaagcat aggaaggctc tatgtttgta tatttctctt tgtcagaata cccctcaact catttgctct aataaatttg actgggttga aaaattaaaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggtgggctttctgcgtttataACTAGTCTGCAG
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J61110 | 1,12 | engineered_region feature/BioBrick |