BBa_K2083000

BBa_K2083000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2083000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Virginia Massa

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 09:32:59

APOBEC1-1X

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2083000_sequence (Version 1)

Description

Coding region for APOBEC1, an enzyme that performs C to U edits on DNA and RNA with a 1xXTEN (44 nucleotide) linker. The one extension linker sequence is TCTGGCTCAGAAACG-CCCGGTACGTCCGAGTCTGCAACACCAGAAAGC. APOBEC with a 2xXTEN is BBa_K2083001, and the 3xXTEN can be found here BBa_K2083002.
Source:
Komor AC et al.Nature. Epub ahead of print (2016)

Notes

We preformed codon optimization to get rid of any EcoRI, XbaI, SpeI, and PstI sites.

Source

This part comes from the sequence of the enzyme in rats.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,3	start_codon, feature/start

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2083000/1

Member of these Collections

• iGEM Parts Registry