Types | DnaRegion
|
Roles | Reporter
engineered_region
|
Sequences | BBa_K2083009_sequence (Version 1)
|
Description
Coding region for a constitutively expressed GFP reporter. A section of the GAPDH gene is used as the 5??? UTR, as it is a known successful target site for Cas9.
Notes
Made sure that there were no EcoR1, Xba1, Spe1, and Pst1 sites.
Source
Uses GFP sequence and part of the GAPDH gene.