ACG GFP

BBa_K2083010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2083010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Virginia Massa

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-15 12:37:28

Mutated Start Codon GFP Reporter

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K2083010_sequence (Version 1)

Description

Coding region for GFP reporter. A section of the GAPDH gene is used as the 5??? UTR, as it is a known successful target site for Cas9 The start codon has been mutated to ACG to decrease, and hopefully prevent translation. Overall, in experiments there was an average of DATA% decreased production of GFP compared to BBa_K2083009. (Chart).

Notes

To decrease translation we mutated the start codon to ACG.

Source

This part comes from GFP and GAPDH sequences.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2083010/1

Member of these Collections

• iGEM Parts Registry