Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nikhil Nair
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-21 08:12:38
atoxic C. difficile toxin B
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2087001_sequence (Version 1) |
Description
NanoLuc, a fluorescent derivative of luciferase, linked via a glycine/serine linker to the mutated glucosyl transferase domain of the C. difficile toxin B (Proven to be safe for humans). The toxin binds to the epithetial cell surface receptors, triggering endocytosis. The toxin then undergoes a conformation change allowing it to escape the lysosome and self cleaves its glucosyltransferase domainNotes
Quite a large protein, so normal E. coli strains might not be able to fold it properly. B. megaterium was used to produce atoxic C. diffile toxin B, so it would be a better bet to produce this protein.Source
From Nanoluc protein and the atoxic mutant of the C. difficile toxin B.| Sequence Annotation | Location | Component / Role(s) |
| Glycine/Serine Linker atoxic C. difficile Toxin B 6x HIS tag | 1,40 41,7171 7172,7192 | sequence_feature feature/misc CDS feature/protein tag feature/tag |