BBa_K2088008

BBa_K2088008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2088008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xia Chen

Date created: 2016-10-07 11:00:00

Date modified: 2016-10-08 09:05:18

catechol 2,3-dioxygenase gene with native rbs

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K2088008_sequence (Version 1)

Description

This part includes catechol 2,3-dioxygenase(C23O) gene from Sphingobium sp. YBL2 and its native ribosome binding site. With a promoter before them, this gene can be used to degrade catechol, yeilding bright yellow product 2-hydroxymuconate semialdehyde with an absorbance maximum around 375 nm. As a result, this part can also be utilized as a reporter.

Notes

None

Source

Sphingobium sp. YBL2. Sequence found from GenBank: http://www.ncbi.nlm.nih.gov/nuccore/NZ_CP010956

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
C23O coding region	36,968	CDS, feature/cds
ATG start codon	36,38	feature/start, start_codon

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2088008/1

Member of these Collections

• iGEM Parts Registry