BBa_K2088692

BBa_K2088692 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2088692
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xia Chen
Date created: 2016-10-19 11:00:00
Date modified: 2016-10-20 11:39:43

gene cluster mhbDHIM



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K2088692_sequence (Version 1)

Description

This gene cluster encodes the enzymes involved in the catabolism of 3-hydroxybenzoate to fumarate and pyruvate via the gentisate pathway.
mhbD, mhbH, mhbI and mhbM genes encode the gentisate 1,2-dioxygenase, maleylpyruvate isomerase, fumarylpyruvate hydratase and 3-HBA 6-hydroxylase respectively.

Notes

There are 3 site ???directed mutagenesis in this gene cluster.

Source

Genes from Klebsiella pneumoniae M5a1.

Sequence Annotation Location Component / Role(s)
native rbs of mhbD
mhbD
native rbs of mhbH
mhbH
native rbs of mhbI
mhbI
native rbs of mhbM
mhbM
1,25
26,1063
1064,1087
1088,1786
1787,1794
1795,2439
2440,2447
2448,3641
ribosome_entry_site feature/rbs
CDS feature/cds
feature/rbs ribosome_entry_site
feature/cds CDS
feature/rbs ribosome_entry_site
feature/cds CDS
feature/rbs ribosome_entry_site
CDS feature/cds
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2088692/1