BBa_K2092000

BBa_K2092000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2092000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Guadalupe Alvarez Gonzalez

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-11 09:11:23

AOx

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2092000_sequence (Version 1)

Description

This part encodes for the AOx1 protein from Pichia Pastoris.

Alcohol oxidase is an oligomeric enzyme with eight identical sub-units, containing a bound molecule cofactor, flavin adenine dinucleotide (FAD). It is produced by methylotrophic yeasts (such us our chosen??Pichia) located and assembled in peroxisomes during growth on methanol (1). AOx is implicated in the methanol oxidation pathway in yeasts, however it can also oxidise other short-chain alcohols, such as ethanol, propanol or butanol (2).

AOX is thus responsible for the oxidation of primary alcohols to the equivalent aldehyde, using molecular oxygen (O2) as the electron acceptor, creating Hydrogen Peroxide (H202) as a by-product.

References:
Azevedo, A. M., Prazeres, D. M. F., Cabral, J. M., & Fonseca, L. P. (2005). Ethanol biosensors based on alcohol oxidase. Biosensors and Bioelectronics,21(2), 235-247.
Maleknia, S., Ahmadi, H., & Norouzian, D. (2011). Immobilization of Pichia pastoris cells containing alcohol oxidase activity. Iranian journal of microbiology, 3(4), 210.

Notes

The part is designed with an NdeI restriction site between the prefix and the start of the AOx gene. This is because we wanted to assemble the gene in protein expression pET vectors, which normally contain an NdeI restriction site after the Ribosome Binding Site.

Source

GenBank

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2092000/1

Member of these Collections

• iGEM Parts Registry