Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hui Wen Chong
Date created: 2016-10-01 11:00:00
Date modified: 2016-10-02 02:03:43
alcR (incl RBS), ethanol-activated transcription factor from A. nidulans
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2092004_sequence (Version 1) |
Description
This part encodes for the alcR gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus Aspergillus nidulans (A.nidulans). It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA (PalcA) and aldA promoters.AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].
Reference:
[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385???396.
Notes
- Codon optimized the sequence retrieved from the NCBI database (original sequence is of eukaryotic origin).- Calculated the RBS strength using the SalisLab RBS Calculator.
| Access | Instance | Definition |
| public public | BBa_J34801 BBa_K2092001 | BBa_J34801 BBa_K2092001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J34801 BBa_K2092001 | 1,12 19,2613 | BBa_J34801 BBa_K2092001 |