BBa_K210001

BBa_K210001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K210001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Makoto Kashima
Date created: 2009-10-08 11:00:00
Date modified: 2015-05-08 01:11:29

lacI repressor with kozak sequence.



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K210001_sequence (Version 1)

    Description

    This part is derived from E.coli's lacI repressor. By PCR, we exchanged ATG to kozak sequence.
    We used this in order to express lacI in yeast.

    Notes

    This part has LVA(rapid degradation tail), but it does not work in yeast.

    Source

    BBa_C0012

    Sequence Annotation Location Component / Role(s)
    kozak
    lacI-LVA
    LVA
    		3,14
    9,1133
    1101,1133
    		ribosome_entry_site feature/rbs
    CDS feature/protein
    feature/tag tag

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K210001/1
     

    Attachments

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