BBa_K2100059

BBa_K2100059 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2100059
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Goupil
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-26 04:54:14

Sensor 9-3p



    • Details

    Types
    DnaRegion

    Roles
    DNA

    sequence_feature

    Sequences BBa_K2100059_sequence (Version 1)

    Description

    This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.

    Notes

    Courtesy of Jeremy Gam of MIT's Weiss Lab

    Source

    Human genome

    Sequence Annotation Location Component / Role(s)
    miR-9-3p-target site
    hEF1a
    eBFP
    hEF1a
    mKate
    		1,88
    1465,2638
    2694,3414
    9034,10207
    10272,10976
    		feature/misc sequence_feature
    feature/promoter promoter
    feature/protein CDS
    promoter feature/promoter
    feature/protein CDS

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2100059/1
     

    Attachments

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