BBa_K210006

BBa_K210006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K210006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Makoto Kashima
Date created: 2009-10-14 11:00:00
Date modified: 2015-05-08 01:11:29

EGFP::PEST sequence generator yeast-optimized



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_K210006_sequence (Version 1)

    Description

    pTEF2(BBa_K165037)+kozak+EGFP+SV40 nuclear localization sequence + ADH1 terminator(BBa_K165018)

    Notes

    We desinged the spencer between EGFP and PEST sequence in order that PEST sequence is translated to correct amino acids.

    Source

    BBa_K165037,BBa_K165018, BBa_E0040

    Sequence Annotation Location Component / Role(s)
    TEF2(BBa_K165037)
    EGFP
    PEST sequence(BBa_K210004)
    BBa_K165018
    		1,404
    418,1136
    1142,1289
    1297,1549
    		feature/promoter promoter
    feature/protein CDS
    tag feature/tag
    polyA_site feature/polya

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K210006/1
     

    Attachments

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