| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K2105000_sequence (Version 1)
|
Description
The lacZ delta M15 mutation encodes a form of beta-galactosidase lacking residues 11-41. This mutation causes it to be inactive until complemented by the lacZ alpha fragment. The lacZ delta M15 mutant is constitutively expressed in DH5 alpha cells. When the delta M15 mutant and the alpha fragment undergo alpha complementation, they are able to cleave the substrate X-gal to produce a blue product.
Notes
The sequence is used unaltered from the 1992 paper by Prentki.
Source
Prentki "Nucleotide sequence of the classical lacZ deletion delta M15" 1992. Gene 122(1):231-2.
