Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maya de Vos
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 10:54:20
YiaT-MCS
| Types | DnaRegion |
| Roles | CDS Coding |
Description
This part contains the membrane protein YiaT (truncated at R232) fused with a multiple cloning site. The multiple cloning site is on the extracellular site of the membrane. This platform allows for the placement of any protein on the outside of the cell.Notes
We ensured that the multiple clonign site did nto contain any restriction sites that were also contained in the suffix and prefix.Source
The sequence for the truncated Yiat protein was derived from:Han, M.J., Lee, S.H. (2014). An efficient bacterial surface display system based on a novel outer membrane anchoring element from the Escherichia coli protein YiaT. FEMS Microbiology Letters, 362, 1-7.