BBa_K2110105

BBa_K2110105 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2110105
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haoran Ding
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-16 01:44:33

PETase site-directed mutant I208V



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K2110105_sequence (Version 1)

Description

This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.

Notes

It should be expressed in Saccharomyces cerevisiae

Source

It is derived from the part BBa_K2110800.

Sequence Annotation Location Component / Role(s)
misc
1,795
sequence_feature feature/misc
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2110105/1