BBa_K2110109

BBa_K2110109 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2110109
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haoran Ding
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-16 01:53:16

PETase site-directed mutant S238F



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K2110109_sequence (Version 1)

    Description

    This is one of the site-directed mutant of the PETase gene, we change the 238th amino acid from A to F. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.

    Notes

    It should be expressed in Saccharomyces cerevisiae.

    Source

    It is derived from the part BBa_K2110800.

    Sequence Annotation Location Component / Role(s)
    misc
    		1,795
    		sequence_feature feature/misc

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2110109/1
     

    Attachments

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