| Types | DnaRegion
|
| Roles | Primer
primer
|
| Sequences | BBa_K2110815_sequence (Version 1)
|
Description
This is the forward for mutant PETase. Site M154 was designed to be replaced by A154. The original codon optimized sequence codes the wild type protein called PETase. PETase is a Poly(ethylene terephthalate) hydrolase, which was found in Ideonella sakaiensis (strain 201-F6) by Japanese scientists. It allows Ideonella sakaiensis to degrade PET. If properly transferred into engineered organisms as E.Coli or yeast, secreted PETase can catalyze the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product. Optimum temperature is 40 degrees Celsius for PET film hydrolysis and optimum pH is 9.
Notes
First apply PCR for mutant section. Then PCR again with original primers of PETase to overlap for the entire mutant PETase.
Source
Artificially Designed
